Part:BBa_K2114006:Design

CotZ_G4S_HA_GST

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 51
  Illegal BamHI site found at 1174
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 571
  

Design Notes

Introduction of HA epitope tag and flexible GGGGS linker by primer extensions and PCR.

Source

The cotZ gene comes from the genome of B. subtilis. The glutathione S-transferase was amplified from the pGEX-6P-1 vector (GE healthcare).